Effect of green tea extract on bonding durability of an etch-and-rinse adhesive system to caries-affected dentin
Journal of Applied Oral Science
J. Appl. Oral Sci. vol.24 no.3 Bauru May./June 2016
J. Appl. Oral Sci. vol.24 no.3 Bauru May./June 2016
http://dx.doi.org/10.1590/1678-775720150518
Objective
Green tea extract has been advocated as a matrix metalloproteinase
(MMP) inhibitor; however, its effect on bond durability to
caries-affected dentin has never been reported. Thus, the aim of this in vitro
study was to evaluate the effect of two MMP inhibitors (2%
chlorhexidine and 2% green tea extract), applied after acid etching, on
bond durability of an etch-and-rinse adhesive system to caries-affected
dentin.
Material and Methods
Occlusal enamel was removed from third molars to expose the dentin
surface, and the molars were submitted to a caries induction protocol
for 15 days. After removal of infected dentin, specimens were
conditioned with 37% phosphoric acid (15 seconds) and randomly divided
into three groups, according to the type of dentin pretreatment (n=10):
NT: no treatment; GT: 2% green tea extract; CLX: 2% chlorhexidine. The
etch-and-rinse adhesive system (Adper™ Single Bond 2, 3M ESPE, St. Paul,
MN, USA) was applied according to the manufacturer's instructions, and
composite resin restorations were built on the dentin. After 24 hours,
at 37°C, the resin-tooth blocks were sectioned perpendicularly to the
adhesive interface in the form of sticks (0.8 mm2 of adhesive
area) and randomly subdivided into two groups according to when they
were to be submitted to microtensile bond strength (μTBS) testing:
immediately or 6 months after storage in distilled water. Data were
reported in MPa and submitted to two-way ANOVA for completely randomized
blocks, followed by Tukey’s test (α=0.05).
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