Effect of green tea extract and mouthwash without alcohol on Candida albicans biofilm on acrylic resin
Interesting how tea can effect denture acrylic MJ
Gerodontology 2014; doi:10.1111/ger.12132 Effect of green tea extract and mouthwash without alcohol on Candida albicans biofilm on acrylic resin
Objective
To evaluate the effect of aqueous extract of green tea and the oral antiseptic without alcohol, on Candida albicans biofilm formation to heat-curing acrylic resin plates.
Background
Candida is associated with oral candidiasis in poorly cleaned dentures.
Material and methods
Standardised specimens of heat-cured (Conv; n = 30) or microwave-cured acrylic resin (Mw; n = 30) were obtained and divided into six groups (n = 10):
G1 = Conv resin and green tea aqueous extract, G2 = Conv resin and
mouthwash, G3 = control of Conv resin, G4 = Mw resin and green tea
aqueous extract, G5 = Mw resin and mouthwash and G6 = control of
Mw-cured resin. The specimens were contaminated with 10 ml of Sabouraud
dextrose broth inoculated with 0.1 ml of standard suspension containing
1 × 106 cells/ml of C. albicans and incubated for
24 h at 37°C. After this period, they were immersed in the aqueous
extract or in mouthwash for 15 min. The control groups were treated with
sterile distilled water. Aliquots of 0.1 ml were plated on Sabouraud
dextrose agar and incubated at 37°C for 24 h. The numbers of
colony-forming units per test specimen (CFU/TS) were calculated, and the
results statistically analysed by two-way anova and Tukey's tests (5%).
Results
Statistically
significant difference was observed for the aqueous extract groups (G1;
33.65%) and mouthwash (G2; 17.06%), when compared to control (G3;
100%), for Conv resin. For the Mw resin, there was significant
difference between mouthwash (G5; 43.16%) and control (G6; 100%).
Conclusion
The
aqueous extract of green tea and mouthwash led to a reduction in the
number of viable fungal cells in biofilm formed on acrylic resin.
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